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plvx ireszsgreen backbone (TaKaRa)

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TaKaRa plvx ireszsgreen backbone
Plvx Ireszsgreen Backbone, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 407 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/backbone+vector/pLVX-IRES-ZsGreen1+Vector/pm41957014-173-10-12
Average 96 stars, based on 407 article reviews

plvx ireszsgreen backbone - by Bioz Stars, 2026-10

96/100 stars

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Plasmid Preparation:

Article Title: Bright multicolor labeling of neuronal circuits with fluorescent proteins and chemical tags
Article Snippet: pCAG-mTurquoise2/EYFP/tdTomato were assembled by using pCAG-CreERT2 (Addgene #14797, RRID: Addgene_14797, from Dr. Cepko), pmTurquoise2-N1 (Addgene #60561, RRID: Addgene_ 60561, from Dr. D. Gadella), EYFP (Clontech), and tdTomato (a gift from Dr. R. Tsien). .. To generate a backbone vector for pTRE-XFP, an XhoXho fragment containing TRE-SV40 poly A was transferred from pTRE-Tight (#631059, Clontech) to pBluescript II SK(+) (# 212205, Agilent) to ensure high-copy expression in Escherichia coli. .. The WPRE sequence was PCR amplified from an aavCAG-pre-mGRASP-mCerulean vector (Addgene #34910, RRID: Addgene_34910, a gift from Dr. J. Kim).

Article Title: Bright multicolor labeling of neuronal circuits with fluorescent proteins and chemical tags
Article Snippet: pCAG-mTurquoise2/EYFP/tdTomato were assembled by using pCAG-CreERT2 (Addgene #14797, RRID: Addgene_14797 , from Dr. Cepko), pmTurquoise2-N1 (Addgene #60561, RRID: Addgene_60561 , from Dr. D. Gadella), EYFP (Clontech), and tdTomato (a gift from Dr. R. Tsien). .. To generate a backbone vector for pTRE-XFP, an Xho-Xho fragment containing TRE-SV40 poly A was transferred from pTRE-Tight (#631059, Clontech) to pBluescript II SK(+) (# 212205, Agilent) to ensure high-copy expression in Escherichia coli . .. The WPRE sequence was PCR amplified from an aavCAG-pre-mGRASP-mCerulean vector (Addgene #34910, RRID: Addgene_34910 , a gift from Dr. J. Kim).

Expressing:

Article Title: Bright multicolor labeling of neuronal circuits with fluorescent proteins and chemical tags
Article Snippet: pCAG-mTurquoise2/EYFP/tdTomato were assembled by using pCAG-CreERT2 (Addgene #14797, RRID: Addgene_14797 , from Dr. Cepko), pmTurquoise2-N1 (Addgene #60561, RRID: Addgene_60561 , from Dr. D. Gadella), EYFP (Clontech), and tdTomato (a gift from Dr. R. Tsien). .. To generate a backbone vector for pTRE-XFP, an Xho-Xho fragment containing TRE-SV40 poly A was transferred from pTRE-Tight (#631059, Clontech) to pBluescript II SK(+) (# 212205, Agilent) to ensure high-copy expression in Escherichia coli . .. The WPRE sequence was PCR amplified from an aavCAG-pre-mGRASP-mCerulean vector (Addgene #34910, RRID: Addgene_34910 , a gift from Dr. J. Kim).

Ligation:

Article Title: Genetically modified non-human animal with human or chimeric LAG3
Article Snippet: .. The pT7-sgRNA vector had a DNA fragment containing the T7 promoter and sgRNA scaffold (SEQ ID NO: 62), and was ligated to the backbone vector (Takara, Catalog number: 3299) by restriction enzyme digestion (EcoRI and BamHI) and ligation. ..